Was this page helpful? Please let us know. The Chameleon Pre-stained Protein Ladders are not standards, and protein band migration may appear different depending on the gel type used.įor instructions on sizing bands in Image Studio™ Software, visit /chameleon-sizing.
The marked bands will chemiluminesce during imaging. For chemiluminescent detection, you may trace the pre-stained bands using a WesternSure Pen prior to the addition of the chemiluminescent substrate.
For individual molecular weights, please refer to the image above. Do not heat above room temperature.Īfter electrophoresis, the ladder will separate into 9 visible bands in the 8 - 260 kDa range. Mix the product thoroughly before use to dissolve any solids that may have precipitated during storage. Just load your samples and reagents into the microplate, and Jess does the rest. Bring the product to room temperature while protecting from light. Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. Use for tracing visible protein ladders or making identifying marks on your blots. A chemiluminescent signal will be detectable from your markings once chemiluminescent substrate is added. A chemiluminescent marker that enables you to write or draw on your transfer membranes. To see a complete portfolio of LI-COR molecular weight markers, visit /proteinmarkers.Ĭhameleon Pre-stained Protein Ladders are provided ready to use in loading buffer. Chemiluminescent pen for blot annotation. The ladder is optimized for use with Bis-Tris and Tris-Glycine systems. The blot was scanned on a C‑DiGit Blot Scanner for 6 minutes.
A distinguishing characteristic is the use of an enzymatic reaction for protein detection. Beware of chemiluminescent Western detection limitations. The example shows a visible image (left) and a chemiluminescent image (right) resolved on a 4 - 12% Bis-Tris Gel and transferred to nitrocellulose via wet tank transfer. Chemiluminescent Western blot detection is commonly-used but indirect and enzymatic. For chemiluminescent detection, annotate the pre-stained bands using the WesternSure® Pen (P/N 926-91000) prior to the addition of substrate. In blotting applications, the ladder can be used to monitor protein transfer and as a reference to estimate the molecular weight of proteins of interest. In gels, the ladder can be used to visualize progress of the protein separation during electrophoresis and to estimate the molecular weight of unknown proteins based on their relative mobility. The Chameleon Vue Pre-stained Protein Ladder provides multicolored, pre-stained, and visible bands of convenient and consistent protein sizes.